Metagenomic next-generation sequencing as an emerging modality … – News-Medical.Net
A recent study published in the Open Forum Infectious Diseases Journal examined the role of plasma metagenomic next-generation sequencing (mNGS), a promising method for detecting infectious illnesses.
Study: Image Credit: ArtemisDiana/Shutterstock.com
Background
The ability to rapidly identify human pathogens in clinical samples has improved due to developments in diagnostic technology for infectious diseases, such as automated methods for microbial identification and matrix-assisted laser desorption ionization-time of flight.
However, these procedures necessitate culture growth; thus, detecting cases where infected material is unavailable, or the etiologic agent cannot be grown in a lab is challenging.
mNGS uses high-throughput sequencing technologies to identify DNA or RNA from suspected pathogens quickly and precisely. NGS can be applied to any patient sample, but microbial cell-free DNA (mcf-DNA) is used for readily obtained samples like patient serum.
Mcf-DNA is released by…
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